Shiitakes, like all mushrooms, produce two spore mating types—the fungal equivalent to sperm and eggs. Once released from the gills of a mature shiitake, spores that find themselves in damp conditions with access to a food supply will germinate and grow to form hyphae.Click to see full answer. Accordingly, how do you propagate Mushroom spores?Remove the stem from the mushroom of your choice and upend it, placing the cap spore side down onto the two pieces of paper with one half on white and one half on black. Cover the mushroom with the glass container to prevent it from drying out.Secondly, how do you get oyster mushroom spores? How to Collect Oyster Mushroom Spores Cut off the stem of the oyster mushroom at the base of the fan-shaped cap. Place the oyster mushroom cap on the dark colored paper with the underside down on the paper. Place the glass cup or bowl over the mushroom cap and place the setup in a cool and dry place for 48 hours. Also question is, how do you get shiitake to spawn? Placing mushroom spores onto a sterile medium to start the fungal growth and then transferring the culture to millet seeds is a good way to make oyster mushroom spawn. Gelatin with a small amount of sugar, boiled for sterilization and poured into small, sterile jars makes a good starting medium.How do you get Portobello Mushroom spores? How to Collect Portobello Spores Choose mushrooms that are still convex or domed on top. Mushrooms that are flat topped or concave may be too dry and over-ripe to produce spores. Cut or pull off the stem and discard. Place the mushroom, gill-side down, on the paper. Remove the bowl and mushroom carefully.