p-Anisaldehyde General purpose stain, particularly good with groups with nucleophilic properties. Add 15 ml of AcOH and 3.5 mL of p- Anisaldehyde to 350 mL ice cold EtOH. Cautiously add 50 mL concentrated H2SO4 dropwise over 60 minutes. Store unused portion at 0°C.Click to see full answer. Besides, how does Anisaldehyde stain work?Anisaldehyde – sulfuric acid is a universal reagent for natural products, that makes color differentiation possible. It tends to stain the TLC plate itself, upon mild heating, to a light pink color, while other functional groups tend to vary with respect to coloration.Additionally, why does the permanganate stain work as a visualization technique? Permanganate Stain Heat may be required to visualize some functional groups, and often improves the contrast between spots and the background. Heating may be done (if needed) until the background color just begins to yellow, but a brown background means the plate was overheated. Similarly, how do you make potassium permanganate stain? Dissolve 1.5g of KMnO4, 10g K2CO3, and 1.25mL 10% NaOH in 200mL water. A typical lifetime for this stain is approximately 3 months. This particular stain is excellent for functional groups whose pKa is approximately 5.0 and lower. Thus, this stain provides an excellent means of selectively visualizing carboxylic acids.How do you stain vanillin? Vanillin Stain Recipe: Add the vanillin to your container followed by ethanol to give a clear solution. Carefully add the sulfuric acid. The final product is a clear colourless solution. However, after some dipping of plates it will quickly become a clear yellow solution (see picture above).
