PCR was invented in 1983 by Kary Mullis. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called Nucleic acid denaturation. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA.Click to see full answer. Thereof, what separates the strands of DNA in the polymerase chain reaction PCR technique?PCR is used to copy just a relatively small region of DNA, not the entire genome. What separates the strands of DNA in the polymerase chain reaction (PCR) technique? Heat. The PCR technique doubles the amount of DNA in a sample in each cycle.Also Know, what is DNA polymerase chain reaction? Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR relies on a thermostable DNA polymerase, Taq polymerase, and requires DNA primers designed specifically for the DNA region of interest. In this manner, what is used to separate the DNA molecule in the PCR process? Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA. Initially, the mixture is heated to denature, or separate, the double-stranded DNA template into single strands. The mixture is then cooled so that the primers anneal, or bind, to the DNA template.How does PCR amplify specific region of DNA?To amplify a segment of DNA using PCR, the sample is first heated so the DNA denatures, or separates into two pieces of single-stranded DNA. Next, an enzyme called “Taq polymerase” synthesizes – builds – two new strands of DNA, using the original strands as templates.