Remember that absorbance is the logarithm of the transmission (T) of light through a sample. Transmission is the ratio of the intensity of light transmitted through the sample (I) to the intensity of light transmitted through a blank (Io). So absorbance = log (Io/I). Any absorbance reading above 1 can be inaccurate.Click to see full answer. In respect to this, what does a high absorbance value indicate?When you get very high absorbance (>1.5), it means that most of the light are absorbed by the sample and only small amount of the light detected by detector.Beside above, what does an absorbance of 1 mean? Measure the transmittance of light. Absorbance can range from 0 to infinity such that an absorbance of 0 means the material does not absorb any light, an absorbance of 1 means the material absorbs 90 percent of the light, an absorbance of 2 means the material absorbs 99 percent of the light and so on. Furthermore, can absorbance values be greater than 1? If the non-linearity occurs at absorbance values higher than one, it is usually better to dilute the sample into the linear portion of the curve because the absorbance value has a high relative error.Why does beer’s law fail at high concentration?The linearity of the Beer-Lambert law is limited by chemical and instrumental factors. Causes of nonlinearity include: deviations in absorptivity coefficients at high concentrations (>0.01M) due to electrostatic interactions between molecules in close proximity. changes in refractive index at high analyte concentration.
